植物花色呈现的生物化学、分子生物学机制及其基因工程改良

赵昶灵 已出版文章查询
赵昶灵
本平台内已出版文章查询
1 郭维明 已出版文章查询
郭维明
本平台内已出版文章查询
2 陈俊愉 已出版文章查询
陈俊愉
本平台内已出版文章查询
3

+ 作者地址

1南京农业大学,生命科学学院,南京,210095

2南京农业大学,园艺学院,南京,210095

3北京林业大学,园林学院,北京,100083


0
  • 摘要
  • 参考文献
  • 相关文章
  • 统计
文章综述了植物花色基因工程改良的前提、策略和特殊性.植物主要花色色素类黄酮、类胡萝卜素的合成途径和相关酶的基因表达已基本研究清楚,关键酶的基因已被克隆.花色的基因工程改良遵循一般植物基因工程规律,而且是系统工程,要求花器官特异性启动子,基本策略为抑制关键酶的基因表达、导入调节基因或新的外源基因.花色基因工程可能成为获得雄性不育的方式之一,应与传统育种技术有机结合,当然也存在安全性问题.

[1] 安田齐;张承志;佟丽.花色之谜[M].北京:中国林业出版社,1989:1-149.

[2] 程金水.园林植物遗传学[M].北京:中国林业出版社,2000:23-207.

[3] 赵云鹏,陈发棣,郭维明.观赏植物花色基因工程研究进展[J].植物学通报,2003(01):51-58.

[4] 何小玲;王金发 .The quality genes and their genetic engineering of ornamental flowers[J].植物生理学通讯,1998,34(06):462-466.

[5] Tanaka Y;TSUDA S;KUSUMI T .Metabolic engineering to modify flower color[J].Plant and Cell Physiology,1998,39:1119-1126.

[6] 郑志亮 .Genetic engineering of flower color of horticulture[J].北方园艺,1994,3:37-38.

[7] 张石宝,胡虹,李树云.花卉基因工程研究进展Ⅰ:花色[J].云南植物研究,2001(04):479-487.

[8] Mol J N M;STUITJE A R;VAN DER KROL A .Genetic manipulation of floral pigmentation genes[J].Plant Molecular Biology,1989,13:287-294.

[9] 薛淮,刘敏,张纯花,潘毅.花卉分子育种研究进展[J].中国生物工程杂志,2002(02):81-84,80.

[10] 彭镇华,汪政科.观赏植物基因工程研究进展[C].中国花卉科技进展(第二届全国花卉科技信息交流会:论文专辑),2001:166-172.

[11] 傅荣昭;孙勇如;贾士荣.植物遗传转化技术手册[M].北京:中国科学技术出版社,1994:42-50.

[12] Mol J;HOLTON T A;KOES R E .Genetic engineering of commercial traits of floral crops[J].Trends in Biotechnology,1995,13:350-355.

[13] Meyer P;HEIDMANN O;FORKMANN G .A new petunia flower color generated by transformation of a mutant with a maize gene[J].NATURE,1987,330:677-678.

[14] 储文华 .Agrobacterium mediated gene transfer to Gypsophila elegans[J].上海师范大学学报(自然科学版),1996,25(04):90-91.

[15] Elomaa P;HONHANEN J;PUSKA R .Agrobacterium mediated transfer of antisense chalcone synthase cDNA to Gerbra hybrida inhibits flower pigmentation[J].Biotechniques,1993,11:508-511.

[16] 张树珍,杨本鹏,刘飞虎.花特异表达启动子PchsA的克隆及其序列分析[J].农业生物技术学报,2002(02):116-119.

[17] 包满珠 .Cloning and application of plant anthocyanin genes[J].园艺学报,1997,24(03):279-284.

[18] BOLWILL G P .L-Phenylalanine ammonialyase from Phaseolus vulgaris:characterization and differential induction of multiple forms from elicitor-treated cell suspension cultures[J].European Journal of Biochemistry,1985,149:411-419.

[19] 林泉.色素基因的表达和调控[A].北京:科学出版社,1998:107-119.

[20] 谢灵玲,赵武玲,沈黎明.光照对大豆叶片苯丙氨酸裂解酶(PAL)基因表达及异黄酮合成的调节[J].植物学通报,2000(05):443.

[21] Douglas C .Structure and elicitor or UV-light stimulated expression of two 4-coumarate:CoA ligase genes in parsley[J].EMBO Journal,1987,6:1189-1195.

[22] Dixon R A;DEY P M;LAMB C J .Phytoalexins:enzymology and molecular biology[J].Advances in Enzymology,1983,55:239-291.

[23] Kreuzaler F;RAGG H;FAUTZ E et al.UV-induction of chalone synthase mRNA in cell suspension cultures of Petrosklinum hortense[J].Proceedings of the National Academy of Sciences of the United States of America,1983,80:2591-2593.

[24] Ursula N K;BARZEN E;BERNHARDT J et al.Chalcone synthase genes in plants:a tool to study evolutionary relationship[J].Journal of Molecular Evolution,1987,26:213-225.

[25] 王金玲;顾红雅.CHS基因的分子进化研究现状[A].北京:高等教育出版社,德国:施普林格出版社,2000:17-24.

[26] Sommer H;SAEDLER H .Structure of the chalcone synthase of Antirrhinum majus[J].Molecular and General Genetics,1986,202:429-434.

[27] Koes R E;SPELT C E;VAN DER ELZEN P J M .Cloning and molecular characterization of the chalcone synthase multigene family of Petunia hybrida[J].Gene,1989,81:245-257.

[28] Koes R E;SPELT C E;MOL J N M .The chalcone synthase multigene family of Petunia hybrida.Gene:Differential light regulated expression during flower development and UV light induction[J].Plant Molecular Biology,1989,30:213-225.

[29] Koes R E;VAN BLOKLAND R;QUATTROCCHIO F et al.Chalcone synthase promoters in petunia are active in pigmented and unpigmented cell types[J].The Plant Cell,1990,2:379-386.

[30] van der Meer I M;SPELT C E;MOL J N M et al.Promoter analysis of the chalcone synthase (CHS A) gene of Petunia hybrida:a 67bp promoter region directs flower-specific expression[J].Plant Molecular Biology,1990,15:95-190.

[31] Schmid J .Developmental and environmental regulation of a bean chalcone synthase promoter in transgenic tobacco[J].The Plant Cell,1990,2:619-631.

[32] Ryder T B;HEDRICK S A;BELL J N et al.Organization and differential activation of a gene family encoding the plant defence enzyme chalcone synthase in Phaseolus vulgris[J].Molecular and General Genetics,1987,210:219-233.

[33] Moustafa E;WONG E .Purification and properties of chalcone-flavanone isomerase from soybean seed[J].Phytochemistry,1967,6:625-632.

[34] Mehdy M C;LAMB C J .Chalcone isomerase cDNA cloning and mRNA induction by fungal elicitor,wounding and infection[J].EMBO Journal,1987,6:1527-1533.

[35] Van Tunen A J;KOES R E;SPELT C E et al.Cloning of two chalcone flavone isomerase genes from Petunia hybrida:coordinate,light-regulated and differential expression of flavonoid genes[J].EMBO Journal,1988,7:1257-1262.

[36] Van Tunen A J;HARTMEN S A;MUR L A et al.Regulation of chalcone flavone isomerase(CHI) gene expression in Petunia hybrida:the use of alternative promoters in corolla,anther and pollen[J].Plant Molecular Biology,1989,12:539-551.

[37] 颜华;宋云;李羽云 et al.Molecular cloning,sequencing of chalcone isomerase gene and its expression in E.coli[J].植物学报,1997,39(11):1030-1034.

[38] 孟繁静.植物花发育的分子生物学[M].北京:中国农业出版社,2000:225-269.

[39] Britsch L;GRISEBACH H .Purification and characterization of (2S)-flavanone-3-hydroxylase from Petunia hybrida[J].European Journal of Biochemistry,1986,156:569-577.

[40] Martin C;CARPENTER R;SOMMER H et al.Control of anthocyanin biosynthesis in flowers of Antirrhinum majus[J].Plant Journal,1991,1:37-49.

[41] Henkel J;FORKMANN G;MIN B W .Anthocyanin biosythesis in distinct genotypes of China aster (Callistephus chinensis)[J].Acta Horticulturae,2000,508:213-214.

[42] Wagner G J .Content and vacuole/extravacuole distribution of neutral sugars,free amino acids and anthocyanin in protoplasts[J].Plant Physiology,1979,64:88-93.

[43] Oreilly C;SHEPHERD N S;PEREIRA A et al.Molecular cloning of the Al locus in Zea mays using the transposable elements En and Mul[J].EMBO Journal,1985,4:877-882.

[44] 吴少华,张大生.花青素生成相关基因dfr研究进展[J].福建林学院学报,2002(02):189-192.

[45] Beld M;MAARTIN C;HUITS H et al.Flavoniod synthesis in Prtunia hybrida:partial characterization of dihydroflavonol-4-feductase genes[J].Plant Molecular Biology,1989,13:491-495.

[46] Britsch L;DEDIO J;SAEDLER H et al.Molecular characterization of flavonone 3β-hydroxylase.Consensus sequence,comparison with related enzymes and the role of conserved histidine residues[J].European Journal of Biochemistry,1993,217:745-754.

[47] Birch RG .Plant transformation: problems and strategies for practical application.[J].Annual Review of Plant Physiology and Plant Molecular Biology,1997(0):297-326.

[48] 王关林;方宏筠.植物基因工程原理与技术[M].北京:科学出版社,1998

[49] HoltonTA;BRUGLIERA F;LESTER D R et al.Cloning and expression of cytochrome P450 genes controlling flower color[J].Nature,1993,366:276-279.

[50] Stotz G;DE VLAMING P;WIERING H et al.Genetic and biochemical studies on flavonoid-3-hydroxylation in flowers of Petunia hybrida[J].Theoretical and Applied Genetics,1985,70:300-305.

[51] Toguri T;UMEMOTO N;KOBAYASHI O et al.Activation of anthocyanin synthesis genes by white light in eggplant hypocotyl tissues,and identification of an inducible P-450 cDNA[J].Plant Molecular Biology,1993,23:933-946.

[52] HoltonTA;TANAKA Y .Blue roses-a pigment of our imagnation[J].Trends in Biotechnology,1994,12:40-42.

[53] MESSON A;HOHMANN S;MARTIN W et al.The En/Spm transposable element of Zea mays contains splice sites at the termini generating a novel intron from a dSpm element in the A2 gene[J].EMBO Journal,1990,9:3051-3058.

[54] FEDEROFF N V;FURTEK D B;NELSON O E .Cloning of the bronze locus in maize by a simple and generalized procedure using the transposable controlling element Activator (Ac)[J].Proceedings of the National Academy of Sciences of the United States of America,1984(87):3825-3829.

[55] Yamazaki M;Yamagishi E;Gong ZZ;Fukuchi-Mizutani M;Fukui Y;Tanaka .Two flavonoid glucosyltransferases from Petunia hybrida: molecular cloning, biochemical properties and developmentally regulated expression[J].Plant Molecular Biology,2002(4):401-411.

[56] KRENZALER F .Flavanone synthase from Petroselium hortense,molecular weight,subunit composition,size of messenger RNA and absence of pantethenyl residue[J].European Journal of Biochemistry,1979,231:99-114.

[57] FULIWARA H;TANAKA V;FUKUI V et al.Anthocyanin saromatic acyltransferase from Gentiana triflora,purification,characterization and its role in anthocyanin biosynthesis[J].European Journal of Biochemistry,1997,249:45-51.

[58] Fujiwara H;Tanaka Y;Yonekura Sakakibara K;Fukuchi Mizutani M;Nakao M;Fukui Y;Yamaguchi .cDNA cloning, gene expression and subcellular localization of anthocyanin 5-aromatic acyltransferase from Gentiana triflora[J].The Plant Journal,1998(4):421-431.

[59] Nakayama T;YONEKURA-SAKAKIBARA K;Sato T et al.Aureusidin synthase:A polyphenol oxidase homolog responsible for flower coloration[J].Science,2000,290:1163-1166.

[60] 安田齐;傅玉兰.花色的生理生物化学[M].北京:中国林业出版社,1989:70-76.

[61] GOODWILL T W.The biochemistry of carotenoids[M].London:Chapman and Hall,1980:120-124.

[62] 徐昌杰,张上隆.植物类胡萝卜素的生物合成及其调控[J].植物生理学通讯,2000(01):64-70.

[63] Moehs CP;Tian L;Osteryoung KW;DellaPenna D .Analysis of carotenoid biosynthetic gene expression during marigold petaldevelopment[J].Plant Molecular Biology,2001(3):281-293.

[64] BRITTON G.Overview of carotenoid biosynthesis[A].Basel:Birkhauser,1998:13-147.

[65] Lichtenthaler HK;Rohmer M;Schwender J .Two independent biochemical pathways for isopentenyl diphosphate and isoprenoid biosynthesis in higher plants.[J].Physiologia plantarum,1997(3):643-652.

[66] Moehs CP;Tian L;Osteryoung KW;DellaPenna D .Analysis of carotenoid biosynthetic gene expression during marigold petaldevelopment[J].Plant Molecular Biology,2001(3):281-293.

[67] DEGBO O;CAMARA B .Purification of isopentenylpyrophosphate isomerase and geranylgeranylpyrophosphate synthase from capsicum chromoplasts by affinity chromatography[J].Biochimica et Biophysica Acta,1987,9:140-148.

[68] Scolnik P A;BARTLEY G E .Nucleotide sequence of an Arabidopsis cDNA for geranylgeranyl pyrophosphate synthase[J].Plant Physiology,1994,104:1469-1470.

[69] Ray J A;BIRD C R;MAUNDERS M et al.Sequence of pTOM5,a ripening related cDNA from tomato[J].Nucleic Acids Research,1987,15:10587.

[70] Lintig J von;Welsch R;Bonk M;Giuliano G;Batschauer A;Kleinig H;Von Lintig J .Light-dependent regulation of carotenoid biosynthesis occurs at the level of phytoene synthase expression and is mediated by phytochrome in Sinapis alba and Arabidopsis thaliana seedlings.[J].The Plant Journal,1997(3):625-634.

[71] Ikoma Y;Komatsu A;Kita M;Ogawa K;Omura M;Yano M;Moriguchi T .Expression of a phytoene synthase gene and characteristic carotenoidaccumulation during citrus fruit development[J].Physiologia plantarum,2001(2):232-238.

[72] Masayuki Kita;Akira Komatsu;Mitsuo Omura;Masamichi Yano;Yoshinori Ikoma;Takaya Moriguchi .Cloning and expression of CitPDS1, a gene encoding phytoene desaturase in citrus[J].Bioscience, Biotechnology, and Biochemistry,2001(6):1424-1428.

[73] Bartley G E;SCOLNIK P A .Molecular biology of carotenoid biosynthesis in plants[J].Annual Review of Plant Physiology and Plant Molecular Biology,1994,45:287-301.

[74] Cunningham F X Jr;POGSON B;Sun Z et al.Functional analysis of the β and ε lycopene cyclase enzymes of Arabidopsis reveals a mechanism for control of cyclic carotenoid formation[J].The Plant Cell,1996,8:1513-1626.

[75] Tian L;DELLAPENNA D .Characterization of a second carotenoid β-hydroxylase gene from Arabidopsis and its relationship to the LUT1 locus[J].Plant Molecular Biology,2001,47:379-388.

[76] Kim IJ;Ko KC;Kim CS;Chung WI .Isolation and characterization of cDNAs encoding beta-carotene hydroxylasein Citrus[J].Plant Science: An International Journal of Experimental Plant Biology,2001(5):1005-1010.

[77] Bouvier F;HUGUENEY P;DHARLINGUE A et al.Xanthophyll biosynthesis in chromoplasts:Isolation and molecular cloning of an enzyme catalyzing the conversion of 5,6-epoxycarotenoid into ketocarotenoid[J].Plant Journal,1994,6:45-54.

[78] Deruere J;BOUVIER F;STEPPUHN J et al.Structure and expression of two plant genes encoding chromoplast-specific proteins:occurrence of partially spliced transcripts[J].Biochemical and Biophysical Research Communications,1994,199:1144-1150.

[79] 徐昌杰,陈大明,张上隆.甜橙辣椒红/辣椒玉红素合成酶同源基因的克隆(简报)[J].实验生物学报,2001(02):147-150.

[80] Bouvier F;d'Harlingue A;Hugueney P;Marin E;Marion Poll A;Camara B .Xanthophyll biosynthesis. Cloning, expression, functional reconstitution, and regulation of beta-cyclohexenyl carotenoid epoxidase from pepper (Capsicum annuum).[J].The Journal of biological chemistry,1996(46):28861-28867.

[81] Katoh A;CHEN A T;MURATA N .Studies on a gene involved in the isomerization from violaxanthin to neoxanthin in Chlamydomonas reinhardtii[J].Plant and Cell Physiology,1997,38(Suppl):118.

[82] Hirschberg J.;Harker M.;Lotan T.;Mann V.;Pecker I.;Cohen M. .MOLECULAR GENETICS OF THE CAROTENOID BIOSYNTHESIS PATHWAY IN PLANTS AND ALGAE[J].Pure and Applied Chemistry,1997(10):2151-2158.

[83] GLENN E B;PABLO A S .Molecular biology of carotenoid biosynthesis in plants[J].Annual Review of Plant Physiology and Plant Molecular Biology,1994,5:287-301.

[84] 蒋迪,徐昌杰,陈大明,张上隆.柑橘转基因研究的现状及展望[J].果树学报,2002(01):48-52.

[85] Forkmann G .Flavonoids as flower pigments:the formation of natural spectrum and its extension by genetic engineering[J].Plant Breeding,1991,106:1-26.

[86] Goodrich J;CARPENTER R;COEN E S .A common regulates pigmentation pattern in diverse plant species[J].CELL,1992,68:955.

[87] Dooner H K;NELSON O E .Interaction among C,R and Vp in the control of the Bz glucosyltransferase during endosperm development in maize[J].GENETICS,1979,91:309.

[88] Sablowski R W M .A flower-specific Myb protein activates transcription of phenylpropanoid biosynthetic genes[J].EMBO Journal,1994,13:128-137.

[89] 余迪求,李宝健.花色素苷生物合成的遗传和发育调控[J].植物生理学通讯,1997(01):71-77.

[90] Goff S A;CONE K C;Fromm M E .Identification of functional domains in the maize transcriptional activator C1:Comparison of wild-type and dominant inhibitor proteins[J].Genes and Development,1991,5:298.

[91] VAN TUNEN A J;MOL J N.Control of flavonoid synthesis and manipulation of flower colour[A].,1991:94-125.

[92] JORGESEN R A .Cosuppression flower color paterns and metastable gene expression states[J].Science,1995,268:686-691.

[93] Timothy A. Holton;Edwina C. Cornish .Genetics and Biochemistry of Anthocyanin Biosynthesis[J].The Plant Cell,1995(7):1071-1083.

[94] Donner H K;ROBBINS T P .Genetic and developmental control of anthocyanin biosynthesis[J].Annual Review of Genetics,1991,25:173-199.

[95] Martin C;PRESCOTT A;MACKAY S et al.Control of anthocyanin biosynthesis in flowers of Antirrhinum mojus[J].Plant Journal,1991,1:37-41.

[96] 瞿礼嘉;顾红雅;胡苹.现代生物技术导论[M].北京:高等教育出版社,1998:241-286.

[97] Mol J;CORNISH E;MASON J et al.Novel coloured flowers[J].Current Opinion in Biotechnology,1999,10:198-201.

[98] VAN DE MEER I M;BROUWER M;SPELT C E et al.The TACPyAT repeats in the chalcone synthase promoter of Petunia hybrida act as a dominant,negative cis-acting module in the control of organ-specific expression[J].Plant Journal,1992,2:525-535.

[99] Zhu CF;Yamamura S;Koiwa H;Nishihara M;Sandmann G .CDNA cloning and expression of carotenogenic genes during flower development in Gentiana lutea[J].Plant Molecular Biology,2002(3):277-285.

[100] 傅荣昭;马江生;曹光诚 et al.Advances in genetic engineering of color,fragrance and shape of ornamental plants[J].园艺学报,1995,22(04):381-385.

[101] Mol J;HOLTON T A;KOES R E .Genetic engineering of commercial traits of floral crops[J].Trends in Biotechnology,1995,13:350-355.

[102] 邵莉;李毅;梁晓文 et al.A new way of flower alteration by expressing chalcone synthase (CHS) gene in transgenic Petunia[J].Bulletin of Biology(生物学通报),1995,30(06):11-12.

[103] Van der Krol A R;MOL J N M;STUITJE A R .Modulation of eukaryotic gene expression by complementary RNA or DNA sequences[J].Biotechniques,1988,6:958-965.

[104] Van der Krol A R;LENTING P E;VEENSTRA J et al.An antisense chalcone synthase gene in transgenic plants inhibits flower pigmentation[J].Nature,1988,333:866-869.

[105] Van der Krol A R;MULR L A;DE LANE P et al.Antisense chalcone synthases genes in petunia:visualization of variable transgene expression[J].Molecular and General Genetics,1990,220:204-210.

[106] Aida R;Kishimoto S;Tanaka Y;Shibata M .Modification of flower color in torenia (Torenia fournieri Lind.) by genetic transformation.[J].Plant Science: An International Journal of Experimental Plant Biology,2000(1):33-42.

[107] Aida R.;Kondo T.;Kishimoto S.;Shibata M.;Yoshida K. .Copigmentation gives bluer flowers on transgenic torenia plants with the antisense dihydroflavonol-4-reductase gene[J].Plant Science: An International Journal of Experimental Plant Biology,2000(1):49-56.

[108] Bird C R;RAY J A;FLETCHER J D et al.Using antisense RNA to study gene function:inhibition of carotenoid biosynthesis in transgenic tomatoes[J].Biotechniques,1991,9:635-639.

[109] BRAMLEY P;TEULIERES C;BLAIN I et al.Biochemical characterization of transgenic tomato plants in which carotenoid synthesis has been inhibited through the expression of antisense RNA to pTOM5[J].Plant Journal,1992,2:343-349.

[110] Que Q;WANG H;ENGLISH J J et al.The frequency and degree of cosuppression by sense chalcone synthase transgenes are depended on transgene promoter strength and are reduced by premature nonsense codoness in the transgene coding sequence[J].The Plant Cell,1997,9:1357-1368.

[111] NAPOLI C;LEMIEUX C;JORENSEN R .Introduction of chimeric chalcone synthase gene into petunia results in reversible co-suppression of homologous genes in transplant[J].The Plant Cell,1990,2:279-289.

[112] Krol A R;MUR L A;BELD M M et al.Flavonoid genes in petunia:addition of a limited number of gene copies may lead to a suppression of gene expression[J].The Plant Cell,1990,2:291-299.

[113] Suzuki K.;Xue HM.;Tanaka Y.;Fukui Y.;Fukuchi-Mizutani M.;Murakami Y. .Flower color modifications of Torenia hybrida by cosuppression ofanthocyanin biosynthesis genes[J].Molecular breeding,2000(3):239-246.

[114] GRAY R G;GRIERSON D .Identification and genetic analysis of normal and mutant phytoene synthase genes of tomato by sequencing,complementation and co-suppression[J].Plant Molecular Biology,1993,22:589-602.

[115] 苏焕然;张丹;汪清胤.Advances in the genetic engineering research of horticulture[J].北方园艺,1996(04):26-28.

[116] Mol J N M;STUITJE A R;VAN DER KROL A .Genetic manipulation of floral pigmentation genes[J].Plant Molecular Biology,1989,13:287-294.

[117] Haseloff J;GERLACH W L .Simple RNA enzymes with new and highly specific endoribonuclease activities[J].NATURE,1988,224:585-591.

[118] Quattrocchio F;WING J F;LEPPON H T C et al.Regulatory genes controlling anthocyanin pigmentation are functionally conserved among plant species and have distinct sets of target genes[J].The Plant Cell,1993,5:1497-1512.

[119] Meyer P;HEIDMANN I;FORKMANN G et al.A new petunia flower color generated by transformation of a mutant with a maize gene[J].NATURE,1985,330:677-678.

[120] Benfey P N;TAKATSUJI H;REN L et al.Sequence requirements of the 5-enolpyruvylshikimate-3-phosphate synthase 5′-upstream region for tissue-specific expression on flowers and seedings[J].The Plant Cell,1990,2:849-856.

[121] Hekariutta Y;ELOMAA P;KOTILAINEN M .Cloning of cDNA coding for dihydroflavonol-4-fedudtase(DFR) an characterization of dfr expression in the corollas of Gerbera hybrida var.regina[J].Plant Molecular Biology,1993,22:163-193.

[122] Fray R G;WALLACE A;FRASER P D et al.Constitutive expression of a fruit phytoene synthase gene in transgenic tomatoes causes dwarfism by redirecting metabolites from the gibberellin pathway[J].Plant Journal,1995,8:693-701.

[123] Kumagai MH;Keller Y;Bouvier F;Clary D;Camara B .Functional integration of non-native carotenoids into chloroplasts by viral-derived expression of capsanthin-capsorubin synthase in Nicotiana benthamiana.[J].The Plant Journal,1998(3):305-315.

[124] Lotan T;Hirschberg J .Cloning and expression in Escherichia coli of the gene encoding beta-C-4-oxygenase, that converts beta-carotene to the ketocarotenoid canthaxanthin in Haematococcus pluvialis.[J].FEBS Letters,1995(2):125-128.

[125] HoltonTA;TANAKA Y .Blue roses-a pigment of our imagination?[J].Trends in Biotechnology,1994,12:40-42.

[126] Mol JNM;Holton TA;Koes RE .FLORICULTURE - GENETIC ENGINEERING OF COMMERCIAL TRAITS[J].Trends in biotechnology,1995(9):350-355.

[127] 于晓南,张启翔.观赏植物的花色素苷与花色[J].林业科学,2002(03):147-153.

[128] 黄胤怡,沈明山,李鹏,陈睦传.蓝色花的基因工程[J].植物生理学通讯,2002(02):203-206.

[129] Shimada Y;Ohbayashi M;Nakano-Shimada R;Okinaka Y;Kiyokawa S .Genetic engineering of the anthocyanin biosynthetic pathway withflavonoid-3 ',5 '-hydroxylase: specific switching of the pathway inpetunia[J].Plant Cell Reports,2001(5):456-462.

[130] Sablowski RWM.;Bevan M.;Baulcombe DC. .EXPRESSION OF A FLOWER-SPECIFIC MYB PROTEIN IN LEAF CELLS USING A VIRAL VECTOR CAUSES ECTOPIC ACTIVATION OF A TARGET PROMOTER[J].Proceedings of the National Academy of Sciences of the United States of America,1995(15):6901-6905.

[131] Solano R;Nieto C;Avila J;Canas L;Diaz I;Paz Ares J .Dual DNA binding specificity of a petal epidermis-specific MYB transcription factor (MYB.Ph3) from Petunia hybrida.[J].EMBO Journal,1995(8):1773-1784.

[132] Linn F;HEIDMANN I;SAEDLER H et al.Epigenetic changes in the expression of the maize A1 gene in Petunia hybrida:role of numbers of integrated gene copies and state of methylation[J].Molecular and General Genetics,1990,222:329.

[133] 邵莉;李毅;杨美珠 et al.Gene expression of chalcone synthase--A (CHS A) in flower color alteration and male sterility transgenic petunia[J].植物学报,1996,38(07):517-524.

[134] 杨朝辉;雷建军;刘翔宇.How do genes regulate flower color[J].植物杂志,2001(02):35-36.

[135] 陶俊,张上隆,徐昌杰,安新民,张良诚.类胡萝卜素合成的相关基因及其基因工程[J].生物工程学报,2002(03):276-281.



期刊热词
  • + 更多
  • 字体大小