高糖干预骨髓来源MAPC细胞周期的研究

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刘泽灏
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李纯
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1中南大学湘雅医院内分泌科,中国湖南长沙,410078


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骨髓来源MPAC是具有多向分化潜能的成体干细胞,高糖是干扰MAPC细胞分化趋势的重要因素.通过流式细胞技术分析高糖对MAPC细胞周期的影响.研究显示,高糖迟滞MAPC细胞的细胞周期在G1/S期.进一步研究的结果提示,高糖可以通过TGF-β1调控ERK1/2信号通路,选择性上调P21蛋白表达,抑制MAPC的细胞周期进程,此时的MAPC细胞Oct4高表达,具有多项分化能力(处于非分化状态).

[1] Chen YH;Guh JY;Chuang TD;Chen HC;Chiou SJ;Huang JS;Yang YL;Chuang LY .High glucose decreases endothelial cell proliferation via the extracellular signal regulated kinase/p15(INK4b) pathway[J].Archives of Biochemistry and Biophysics,2007(1):164-171.

[5] Ruscetti FW;Akel S;Bartelmez SH .Autocrine transforming growth factor-beta regulation of hematopoiesis: many outcomes that depend on the context.[J].Oncogene,2005(37):5751-5763.

[6] Boumaza I;Srinivasan S;Witt WT;Feghali-Bostwick C;Dai Y;Garcia-Ocana A;Feili-Hariri M .Autologous bone marrow-derived rat mesenchymal stem cells promote PDX-1 and insulin expression in the islets, alter T cell cytokine pattern and preserve regulatory T cells in the periphery and induce sustained normoglycemia.[J].Journal of Autoimmunity,2009(1):33-42.

[9] Ivanova N;Dobrin R;Lu R;Kotenko I;Levorse J;DeCoste C;Schafer X;Lun Y;Lemischka IR .Dissecting self-renewal in stem cells with RNA interference[J].Nature,2006(7102):533-538.

[10] Niwa H;Miyazaki J;Smith AG .Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells (see comments)[J].Nature Genetics,2000(4):372-376.

[13] ?ustá?ková,G.;Legartová,S.;Kozubek,S.;Stixová,L.;Pacherník,J.;Bártová,E. .Differentiation-independent fluctuation of pluripotency-related transcription factors and other epigenetic markers in embryonic stem cell colonies[J].Stem cells and development,2012(5):710-720.

[15] Breyer,A;Estharabadi,N;Oki,M;Ulloa,F;Nelson-Holte,M;Lien,L;Jiang,Y .Multipotent adult progenitor cell isolation and culture procedures.[J].Experimental Hematology,2006(11):1596-1601.

[18] Huijberts MS;Schaper NC;Schalkwijk CG .Advanced glycation end products and diabetic foot disease.[J].Diabetes/metabolism research and reviews,2008(Suppl.1):S19-24.

[19] Qi Xiaodan;Fang Shaohong;Jin Yuhong;Han Xiaoying;Wang Yi;Wang Aimin;Zhou Hongbo .Carnosine inhibits high glucose-induced mesangial cell proliferation through mediating cell cycle progression.[J].Regulatory Peptides: An International Journal,2009(1/3):69-76.

[20] Fujii S;Maeda H;Tomokiyo A;Monnouchi S;Hori K;Wada N;Akamine A .Effects of TGF-beta1 on the proliferation and differentiation of human periodontal ligament cells and a human periodontal ligament stem/progenitor cell line.[J].Cell and Tissue Research,2010(2):233-242.

[24] Wu D;Peng F;Zhang B;Ingram AJ;Kelly DJ;Gilbert RE;Gao B;Krepinsky JC .PKC-beta1 mediates glucose-induced Akt activation and TGF-beta1 upregulation in mesangial cells.[J].Journal of the American Society of Nephrology: JASN,2009(3):554-566.

[26] Kim YH;Ryu JM;Lee YJ;Han HJ .Fibronectin synthesis by high glucose level mediated proliferation of mouse embryonic stem cells: Involvement of ANG II and TGF-beta1.[J].Journal of Cellular Physiology,2010(2):397-407.

[27] Mahmoud M;Upton PD;Arthur HM .Angiogenesis regulation by TGFbeta signalling: clues from an inherited vascular disease.[J].Biochemical Society Transactions,2011(6):1659-1666.

[29] Kim YH;Heo JS;Han HJ .High glucose increase cell cycle regulatory proteins level of mouse embryonic stem cells via PI3-K/Akt and MAPKs signal pathways.[J].Journal of Cellular Physiology,2006(1):94-102.

[30] Hayashida T;Schnaper HW .High ambient glucose enhances sensitivity to TGF-beta1 via extracellular signal--regulated kinase and protein kinase Cdelta activities in human mesangial cells.[J].Journal of the American Society of Nephrology: JASN,2004(8):2032-2041.

[32] Salomoni, Paolo;Calegari, Federico .Cell cycle control of mammalian neural stem cells: putting a speed limit on G1[J].Trends in Cell Biology,2010(5):233-243.

[33] Luo,M.;Liu,Z.;Chen,G.;Hao,H.;Lu,T.;Cui,Y.;Lei,M.;Verfaillie,C.M. .High glucose enhances TGF-β1 expression in rat bone marrow stem cells via ERK1/2-mediated inhibition of STAT3 signaling[J].Life sciences,2012(13/14):509-518.


语种: 中文   

基金湖南省科学技术厅科技计划一般项目(2012fj4352)

关键词高糖 MAPC细胞 细胞周期 TGF-β1 P21 ERK1/2


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